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Updated: Feb 16, 2026

The Lambda Select cII Mutation Detection System
Published on: April 26, 2018
Detection of Bax Microsatellite Mutations and BaxΔ2 Isoform in Human Buccal Cells
Honghong Zhang1, Cecilie Tassone1, Nora Lin1
1Department of Biology, Illinois Institute of Technology, 3101 South Dearborn Street, Chicago, IL 60616.
Abstract:
Loss of the pro-apoptotic Bcl-2 family protein Bax occurs in ~50% of hereditary nonpolyposis colorectal cancer (HNPCC) due to microsatellite instability (MSI). Recently, we found that some of the "Bax-negative" MSI tumor cells contain a functional Bax isoform, BaxΔ2, which sensitizes cells to selective chemotherapeutics. Here we show the detection of Bax microsatellite mutations and expression of BaxΔ2 proteins in human buccal cells. Our study provides a sensitive and non-invasive approach and a potential clinical application in diagnosis and treatment of MSI colon cancer patients.
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