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Published on: January 30, 2017
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Function of JARID2 in bovines during early embryonic development
Yao Fu1, Jia-Jun Xu1, Xu-Lei Sun1
1Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, Jilin, China.
Peerj
|January 6, 2018
Summary
JARID2 is crucial for early bovine embryonic development, regulating key genes like OCT4 and SOX2 by modifying H3K27me3. Its expression pattern in oocytes and embryos provides insights into epigenetic regulation.
Area of Science:
- Epigenetics
- Developmental Biology
- Reproductive Science
Background:
- Histone lysine modifications are vital epigenetic regulators in early embryonic development.
- JARID2, a jumonji demethylase family member, influences embryonic development and stem cell differentiation by modifying histone lysines.
- JARID2's role in regulating H3K27me3 methylation is critical for normal early embryonic development.
Purpose of the Study:
- To investigate the expression pattern of JARID2 in bovine oocytes and early embryonic stages.
- To determine the effect of JARID2-induced H3K27 methylation on bovine early embryonic development.
- To elucidate the molecular pathway through which JARID2 regulates H3K27me3 in early embryonic development.
Main Methods:
- Quantitative reverse transcription PCR (qRT-PCR) to detect JARID2 mRNA expression.
- Immunofluorescence to determine JARID2 localization in bovine oocytes and embryos.
- JARID2 knockdown using siRNA microinjection followed by qRT-PCR analysis of developmental genes (OCT4, SOX2, c-myc).
Main Results:
- JARID2 mRNA is highly expressed in various bovine oocyte and embryonic stages (GV, MII, 2-cell, 4-cell, 8-cell, 16-cell, blastocyst), with significantly lower expression in GV oocytes.
- JARID2 is primarily localized in the nucleus.
- JARID2 knockdown led to significantly increased expression of OCT4, SOX2, and c-myc in 2-cell embryos compared to blastocysts.
Conclusions:
- JARID2 exhibits a distinct expression pattern throughout bovine oocyte and early embryonic development.
- JARID2 plays a significant role in early bovine embryonic development by regulating the expression of OCT4, SOX2, and c-myc through H3K27me3 modification.
- Findings offer insights for improving in vitro embryo culture efficiency and understanding early embryonic development regulation.
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