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Levels of persisters influenced by aeration in Acinetobacter calcoaceticus-baumannii

Bruna Kern Donamore1, Stephanie Wagner Gallo1, Pedro Maria Abreu Ferreira2

  • 1PUCRS, Escola de Ciências, Laboratório de Imunologia e Microbiologia, Porto Alegre, RS, Brazil.

Future Microbiology
|January 6, 2018
PubMed
Abstract

Insights

Aeration significantly reduced persister cells of Acinetobacter calcoaceticus-baumannii when exposed to antibiotics like meropenem and tobramycin. This suggests aeration impacts bacterial survival strategies against antimicrobial treatments.

Area of Science:

  • Microbiology
  • Antimicrobial Resistance
  • Bacterial Physiology

Background:

  • Persister cells are a subpopulation of microorganisms exhibiting temporary resistance to antibiotics.
  • Understanding factors influencing persister cell formation is crucial for combating persistent infections.

Purpose of the Study:

  • To investigate the effect of aeration on persister cell levels in Acinetobacter calcoaceticus-baumannii.
  • To analyze morphological and structural alterations in persister cells under different conditions.

Main Methods:

  • Acinetobacter calcoaceticus-baumannii isolates were exposed to meropenem or tobramycin.
  • Persister cell levels were quantified after 48-hour exposure under aerated and static conditions.
  • Scanning and transmission electron microscopy were employed to examine persister cell morphology.

Main Results:

  • Persister fractions varied among different bacterial isolates.
  • Aeration led to a reduction in cell survival during antibiotic treatment.
  • Increased tobramycin concentration correlated with decreased cell survival.
  • Electron microscopy revealed the presence of division septa in persister cells.

Conclusions:

  • Aeration may enhance antibiotic efficacy by stimulating bacterial growth and increasing antibiotic targets.
  • Aeration might also promote reactive oxygen species production, contributing to reduced persister levels.
  • Aeration is a potential factor influencing the survival of Acinetobacter calcoaceticus-baumannii persister cells.
Keywords:
Acinetobacter spp.meropenemoxygen availabilitypersistencetobramycin

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