Related Experiment Video

Updated: Feb 16, 2026

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.8K

MicroRNA-187 Inhibits Growth and Metastasis of Osteosarcoma by Downregulating S100A4

Yi Xiao1, Quan Zhao2, Bo Du3

  • 1a Department of Orthopaedic , The People's Hospital of Kaizhou District , Chongqing , PR China.

Cancer Investigation
|January 6, 2018
PubMed

Insights

MicroRNA-187 (miR-187) is downregulated in osteosarcoma (OS). Restoring miR-187 inhibits OS cell growth and metastasis by targeting S100A4, suggesting its potential as a therapeutic target.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) play crucial roles in cellular processes.
  • Dysregulated miRNA expression is implicated in various cancers, including osteosarcoma (OS).
  • Specific miRNAs may serve as diagnostic biomarkers or therapeutic targets for OS.

Purpose of the Study:

  • To investigate the role of miR-187 in osteosarcoma.
  • To identify the molecular mechanisms underlying miR-187's function in OS.
  • To evaluate miR-187 as a potential therapeutic target for OS.

Main Methods:

  • Quantitative real-time PCR to assess miR-187 expression levels in OS tissues and cell lines.
  • In vitro experiments involving miR-187 overexpression and knockdown in U-2OS cells to evaluate proliferation and metastasis.
  • In vivo studies using mouse models to assess the effect of miR-187 on OS tumor growth and metastasis.
  • Luciferase reporter assays to confirm the interaction between miR-187 and the S100A4 3'-UTR.

Main Results:

  • miR-187 expression was significantly decreased in OS tissues and cell lines compared to normal controls.
  • Overexpression of miR-187 inhibited proliferation and metastasis of OS cells in vitro.
  • Knockdown of miR-187 promoted proliferation and metastasis of OS cells in vitro.
  • miR-187 suppressed OS cell growth and metastasis in vivo.
  • miR-187 directly targets the 3'-UTR of S100A4, leading to its downregulation in OS cells.

Conclusions:

  • miR-187 functions as a tumor suppressor in osteosarcoma.
  • miR-187 inhibits osteosarcoma growth and metastasis by downregulating S100A4 expression.
  • miR-187 holds potential as a diagnostic biomarker and therapeutic target for osteosarcoma.
Keywords:
OsteosarcomaProliferationS100A4metastasismiR-187

More Related Videos

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

11.6K
Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay07:44

Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay

Published on: March 12, 2018

10.7K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.1K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.9K
Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
57.4K
Metastasis02:30

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
6.6K
Enzyme Inhibition01:30

Enzyme Inhibition

Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
92.9K