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Hemodynamic effects on endothelial cell monolayer detachment from vascular prostheses
H P Greisler1, E D Endean, J J Klosak
1Department of Surgery, Loyola University Medical Center, Maywood Ill 60153.
Archives of Surgery (Chicago, Ill. : 1960)
|April 1, 1989
Summary
Endothelial cells adhered well (90%) to Hytrel grafts under simulated blood flow. This suggests endothelialized surfaces can improve graft integration, with minimal cell loss after initial perfusion.
Area of Science:
- Biomaterials Science
- Vascular Biology
- Regenerative Medicine
Background:
- Endothelialized surfaces on vascular grafts may enhance graft-host integration.
- Polyester elastomer (Hytrel) is a potential biomaterial for vascular grafts.
Purpose of the Study:
- To evaluate the adherence of endothelial cells cultured on Hytrel grafts under simulated physiological conditions.
- To assess the impact of hemodynamic shear stress on endothelial cell retention on Hytrel grafts.
Main Methods:
- Indium 111-radiolabeled endothelial cells were cultured to confluence on fibronectin-treated Hytrel grafts.
- Grafts were perfused for two hours using a pulse duplicator under high- and low-shear conditions.
- Radioactivity in perfusate and graft segments was measured to determine cell adherence and retention.
Main Results:
- Excellent endothelial cell adherence (90%) was maintained on Hytrel grafts after 120 minutes of perfusion.
- Most cell loss occurred within the initial 15 minutes of perfusion.
- No significant differences in cell adherence were observed between high- and low-shear conditions or across different graft segments.
Conclusions:
- Endothelial cell adherence to fibronectin-coated Hytrel grafts is robust under simulated hemodynamic conditions.
- Hytrel grafts demonstrate potential for supporting endothelialized surfaces, crucial for improving vascular graft performance.
- The findings support the use of endothelialized grafts to enhance the graft-host relationship in vascular surgery.