Sphingobacterium terrae sp. nov., isolated from oil-contaminated soil

A cream-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, designated strain Brt-F^T, was isolated from oil-contaminated soil. Strain Brt-F^T was able to grow from 15 to 45 °C, pH 6.5-10.5 and 0-4.5 % (w/v) NaCl concentration. This strain was taxonomically characterized by a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain Brt-F^T represented a member of the genus Sphingobacterium and shared highest sequence similarity with Sphingobacterium chuzhouense DH-5^T (99.4 %); Sphingobacterium gobiense H7^T (95.7 %) and Sphingobacterium arenae H-12^T (94.3 %). The only respiratory quinone was menaquinone-7, the major polar lipid was phosphatidylethanolamine and the predominant fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0, and iso-C17 : 0 3-OH. The DNA G+C content was 43.4 mol%. The DNA-DNA relatedness value between strain Brt-F^T and S. chuzhouense KCTC 42746^T was 35.7 %, which falls below the threshold value of 70 % for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain Brt-F^T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacteriumterrae sp. nov. is proposed. The type strain is Brt-F^T (=KEMB 9005-691^T=KACC 19392^T=JCM 32159^T).