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Updated: Feb 16, 2026

Serial Two-Photon Tomography of the Whole Marmoset Brain for Neuroanatomical Analyses
Published on: January 17, 2025
Oligoethyleneoxy-Modified 99mTc-Labeled β-Amyloid Imaging Probes with Improved Brain Pharmacokinetics for
Xiaoyang Zhang1, Yaqin Hou2, Cheng Peng2
1Key Laboratory of Radiopharmaceuticals, Ministry of Education, Beijing Normal University , Beijing 100875, China.
Abstract:
An oligoethyleneoxy linker was introduced for conjugation between 99mTc/Re-bis(aminoethanethiol) (BAT) and β-amyloid (Aβ) binding scaffolds. Rhenium complexes exhibited high to moderate binding affinity to Aβ1-42 aggregates and efficient fluorescent staining to Aβ plaques in brain tissue. After radiolabeling, the 99mTc-labeled probes revealed improved brain pharmacokinetics in normal ICR mice. Probe [99mTc]15 with potent binding affinity (Ki = 13.4 nM) and the highest initial brain uptake (2.10% ID/g at 2 min) in normal ICR mice was evaluated further. In vitro autoradiography showed specific labeling of Aβ plaques by [99mTc]15 in transgenic (Tg) mouse brain tissue. Ex vivo autoradiography further demonstrated its efficient labeling of Aβ plaques in a living Tg mouse. In vivo single photon emission computed tomography (SPECT)/CT imaging in six rhesus monkeys revealed remarkably improved brain uptakes (1.94-2.63% ID within 20 min) of [99mTc]15, making it highly potential to be used in humans for Aβ plaques imaging in the brain.
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