In Vivo Electron Paramagnetic Resonance: Radical Concepts for Translation to the Clinical Setting

Electron paramagnetic resonance (EPR)-based spectroscopic and imaging techniques allow for the study of free radicals-molecules with one or more unpaired electrons. Biological EPR applications include detection of endogenous biologically relevant free radicals as well as use of specially designed exogenous radicals to probe local microenvironments. This Forum focuses on recent advances in the field of in vivo EPR applications discussed at the International Conference on Electron Paramagnetic Resonance Spectroscopy and Imaging of Biological Systems (EPR-2017). Although direct EPR detection of endogenous free radicals such as reactive oxygen species (ROS) in vivo remains unlikely in most cases, alternative approaches based on applications of advanced spin traps and probes for detection of paramagnetic products of ROS reactions often allow for specific assessment of free radical production in living subjects. In recent decades, significant progress has been achieved in the development and in vivo application of specially designed paramagnetic probes as "molecular spies" to assess and map physiologically relevant functional information such as tissue oxygenation, redox status, pH, and concentrations of interstitial inorganic phosphate and intracellular glutathione. Recent progress in clinical EPR instrumentation and development of biocompatible paramagnetic probes for in vivo multifunctional tissue profiling will eventually make translation of the EPR techniques into clinical settings possible. Antioxid. Redox Signal. 28, 1341-1344.