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Dendrotoxin acceptor from bovine synaptic plasma membranes. Binding properties, purification and subunit composition
1Department of Biochemistry, Imperial College, London, U.K.
The Biochemical Journal
|February 1, 1989
Abstract:
Dendrotoxin is a snake polypeptide that blocks selectively and potently certain voltage-sensitive, fast-activating K+ channels in the nervous system, where it binds with high affinity to membranous acceptors. Herein, the acceptor protein for dendrotoxin in bovine synaptic membranes is solubilized in active form and its complete purification achieved by affinity chromatography, involving a novel elution procedure. This putative K+-channel constituent is shown to be a large oligomeric glycoprotein containing two major subunits, with Mr values of 75,000 and 37,000.