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Updated: Feb 16, 2026

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment
Published on: September 14, 2013
National Healthcare Safety Network laboratory-identified Clostridium difficile event reporting: A need for diagnostic
Clare Rock1, Zoi Pana2, Surbhi Leekha3
1Department of Medicine, Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, MD; Armstrong Institute for Patient Safety and Quality, Johns Hopkins University School of Medicine, Baltimore, MD; Department of Hospital Epidemiology and Infection Control, Johns Hopkins Hospital, Baltimore, MD.
Abstract:
We describe the proportion of health care facility-onset Clostridium difficile infection (HO-CDI) National Healthcare Safety Network laboratory-identified events at our facility that were deemed nontrue HO-CDIs. Reasons included testing in a patient without significant diarrhea or with recent laxative use, or delayed testing. Standardized infection ratios using only true HO-CDI in the numerator were improved compared with publically reported standardized infection ratios. A prioritization matrix identifies which clinical services could benefit most from directed diagnostic stewardship interventions.
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