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Updated: Feb 16, 2026

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells
08:28

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

Published on: May 9, 2025

768

Production of human single-chain fragment antibody (ScFv) against human epidermal growth factor receptor-2 (HER-2) by

Saeideh Foroumadi1, Masoumeh Rajabibazl2,3, Azam Rahimpour4

  • 1Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

In Vitro Cellular & Developmental Biology. Animal
|January 7, 2018
PubMed

Insights

Researchers isolated anti-HER-2 single-chain fragment antibodies (ScFvs) using cell panning. This method proved more effective for identifying ScFvs targeting HER-2+ breast cancer cells.

Area of Science:

  • Oncology
  • Immunology
  • Biotechnology

Background:

  • Breast cancer is a leading global cancer in women, with 20% experiencing progression or metastasis despite treatments.
  • Human epidermal growth factor receptor-2 (HER-2) overexpression defines aggressive HER-2+ breast cancer, often resistant to therapy.
  • Single-chain fragment antibodies (ScFvs) offer improved tumor penetration compared to conventional antibodies.

Purpose of the Study:

  • To isolate human single-chain fragment antibodies (ScFvs) targeting the HER-2 receptor.
  • To compare the efficacy of cell panning versus mix antigen-cell panning for ScFV isolation.

Main Methods:

  • Utilized a human ScFv antibody library for isolation.
  • Employed cell panning and mix antigen-cell panning strategies.
  • Analyzed binding activity and specificity of isolated ScFvs against HER-2-expressing cells and recombinant HER-2.

Main Results:

  • Successfully isolated anti-HER-2 ScFv antibody fragments.
  • Demonstrated the superior efficiency of the cell panning strategy for ScFV isolation.
  • Confirmed binding activity and specificity of isolated ScFvs.

Conclusions:

  • Cell panning is a highly efficient strategy for isolating specific ScFvs against cell surface targets like HER-2.
  • The isolated anti-HER-2 ScFvs hold potential for targeted breast cancer therapy.
  • ScFvs represent a promising therapeutic modality for HER-2+ breast cancer due to enhanced tumor penetration.
Keywords:
HER-2Phage displayRecombinant antibodySingle-chain fragment antibodyTumor marker

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