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Single-Walled Carbon Nanotubes Probing the Denaturation of Lysozyme
Liming Xie1, Shin G Chou1, Ajay Pande1
1Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China, Pfizer Global Research and Development, Pfizer Inc., Groton, Connecticut 06340, Optical Technology Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, Chemistry Department, University at Albany, State University of New York, Albany, New York 12222, and Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
Abstract:
Resonance Raman spectroscopy measurements of lysozyme-bound single-walled carbon nanotubes have been made during different stages of the chemically and thermally induced misfolding and of the denaturation process of nanotube-bound lysozymes. Changes to the Raman intensity of single-walled carbon nanotubes (SWNTs) have been observed during the denaturation of lysozyme. The Raman intensity changes are attributed to excitonic transition energy (E ) shifts of the SWNTs during the denaturation of lysozyme. The E shift of SWNTs was confirmed by photoluminescence measurements.
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