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Time-related shape control modifications during erythrocyte storage with additive solutions.
V De Angelis1, B M Orazi, L Santarossa
1Centro Immuno-Trasfusionale, Ospedale Civile di Pordenone, Italy.
Blut
|March 1, 1989
Summary
Red blood cell shape recovery after chemical alteration is possible for up to five weeks during blood bank storage. Storage reduces this capability, but it is not entirely lost, suggesting reversible shape perturbations.
Area of Science:
- Hematology
- Biophysics
- Blood banking
Background:
- Erythrocyte shape is critical for blood flow and oxygen transport.
- Blood bank storage can induce shape alterations in red blood cells (erythrocytes).
- Understanding the reversibility of these shape changes is crucial for transfusion efficacy.
Purpose of the Study:
- To investigate the reversibility of shape alterations in erythrocytes stored under blood bank conditions.
- To assess shape recovery after chemical crenation and rheological properties over a five-week storage period.
- To explore potential mechanisms influencing shape recovery during storage.
Main Methods:
- Studied shape recovery after chemical crenation in erythrocyte concentrates preserved with PAGGS-sorbitol.
- Evaluated rheological properties during a five-week storage period under blood bank conditions.
- Correlated red cell adenosine triphosphate (ATP) content with shape recovery and viscosity.
Main Results:
- Red cell capability to regain normal discoid shape decreases during storage but remains over five weeks.
- A significant, weak correlation was found between red cell ATP content and both shape recovery and viscosity.
- Shape perturbations following blood bank storage are largely reversible.
Conclusions:
- Red blood cell shape alterations during blood bank storage are widely reversible.
- Two mechanisms, ATP-dependent and energy-independent, may reduce shape recovery capability.
- Maintaining higher energy levels during storage could preserve the energy-dependent mechanism for shape recovery.