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Semaphorin-3A as An Immune Modulator Is Suppressed by MicroRNA-145-5p

Mahsa Rezaeepoor1, Mazdak Ganjalikhani-Hakemi1, Shima Shapoori1

  • 1Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Cell Journal
|January 9, 2018
PubMed
Abstract

Insights

MicroRNA-145-5p (miR-145-5p) reduces Semaphorin-3A (SEMA3A) expression in immune cells. This finding highlights SEMA3A

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Semaphorin-3A (SEMA3A) and its receptors are present on immune cells, regulating immune cell over-activation.
  • Down-regulation of SEMA3A is observed in certain autoimmune diseases.
  • Bioinformatic predictions suggested a regulatory role for miR-145-5p in SEMA3A expression.

Purpose of the Study:

  • To investigate the effect of miR-145-5p on SEMA3A gene expression levels.
  • To validate bioinformatic predictions regarding miR-145-5p's influence on SEMA3A.

Main Methods:

  • Transfection of miR-145-5p into peripheral blood mononuclear cells (PBMCs).
  • Quantification of SEMA3A expression using Enzyme-Linked Immunosorbent Assay (ELISA) for protein levels.
  • Quantitative real-time polymerase chain reaction (PCR) for mRNA levels.

Main Results:

  • miR-145-5p significantly decreased SEMA3A expression at both the protein and mRNA levels.
  • Experimental results confirmed the bioinformatic prediction of miR-145-5p's inhibitory effect on SEMA3A.

Conclusions:

  • miR-145-5p acts as a negative regulator of SEMA3A expression in immune cells.
  • These findings shed light on a previously unknown aspect of SEMA3A's role in autoimmune disorders such as multiple sclerosis (MS) and rheumatoid arthritis (RA).
  • SEMA3A is proposed as a potential therapeutic target for autoimmune diseases.
Keywords:
Semaphorin-3AmiR-145-5pmicroRNAs

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