Related Experiment Video

Updated: Feb 16, 2026

A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures
05:02

A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures

Published on: February 10, 2011

25.0K

Immunological responses against vancomycin-resistant Enterococcus faecium and Enterococcus faecalis by mice

Yun Sang Cho1, Mun Han Lee2, Jong Man Kim1

  • 1a Bacterial Disease Division, Animal and Plant Disease Research Department , Animal and Plant Quarantine Agency , Gimcheon , Gyeongsangbuk-do , Republic of Korea.

Journal of Immunoassay & Immunochemistry
|January 9, 2018
PubMed

Insights

Researchers identified specific proteins from vancomycin-resistant Enterococcus (VRE) strains that trigger an immune response in mice. These immunogenic VRE proteins could aid in developing new diagnostic tools and treatments for VRE infections.

Area of Science:

  • Microbiology
  • Immunology
  • Infectious Diseases

Background:

  • Methicillin-resistant Staphylococcus aureus (MRSA) treatment relies solely on vancomycin.
  • Vancomycin-resistant Enterococcus (VRE) poses a significant public health threat as MRSA can acquire vancomycin resistance.
  • Current diagnostic tools for VRE are limited, despite extensive research on their microbiology and genetics.

Purpose of the Study:

  • To identify immunogenic proteins from VanA and VanB classes of VRE.
  • To explore potential targets for serological diagnostic tools and immunotherapeutic VRE antibodies.

Main Methods:

  • Mice were orally infected with VanA (E. faecium) or VanB (E. faecalis) strains.
  • Proteins were separated using SDS-PAGE and identified via Western blot analysis.
  • Serological immune responses to specific VRE proteins were analyzed post-infection.

Main Results:

  • VanA infection elicited immune responses to 27 and 65 kDa cell envelope (CE) proteins at 1 week post-infection (wpi), and a 100 kDa cytoplasmic protein (CP) at 2-4 wpi.
  • VanB infection induced responses to 70 kDa CE protein (1-4 wpi), 25 and 35 kDa CE proteins (3-4 wpi), 79 kDa CE protein (4 wpi), and a 39 kDa CP (3 wpi).

Conclusions:

  • Specific immunogenic proteins from VanA and VanB VRE strains have been identified.
  • These identified proteins show potential for developing novel serological diagnostic methods for VRE.
  • The findings may facilitate the production of VRE-specific immunotherapeutic antibodies.
Keywords:
VanAVanBVancomycin-resistant enterococcicell envelope proteincytoplasmic proteinimmunologic response

More Related Videos

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters12:14

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

Published on: March 9, 2016

10.3K
Treatment with Vancomycin Loaded Calcium Sulphate and Autogenous Bone in an Improved Rabbit Model of Bone Infection09:09

Treatment with Vancomycin Loaded Calcium Sulphate and Autogenous Bone in an Improved Rabbit Model of Bone Infection

Published on: March 14, 2019

9.4K

Related Concept Videos

Immunological Memory01:23

Immunological Memory

Immunological memory, a pivotal pillar of the adaptive immune system, is responsible for the body's ability to remember and respond more swiftly and effectively to previously encountered pathogens. This remarkable feature is what makes vaccines so effective in preventing diseases.
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature...
17.1K
Resistivity01:22

Resistivity

When a voltage is applied to a conductor, an electrical field is generated, and charges in the conductor feel the force due to the electrical field. The current density that results depends on the electrical field and the properties of the material. In some materials, including metals at a given temperature, the current density is approximately proportional to the electrical field. In these cases, the current density can be modeled as:
4.6K
Resistance01:19

Resistance

When a current moves through any conductor, the conductor causes some level of difficulty for the current to flow. The measure of that difficulty is known as the resistance of the material and is represented by R. Every material has its own resistance. In the case of conductors, heat is emitted whenever a current passes through them. Resistance depends on the resistivity of the material. Resistivity is a characteristic of the material used to fabricate electrical components, whereas the...
6.1K
Equivalent Resistance01:16

Equivalent Resistance

In circuit analysis, situations often arise where resistors are neither in series nor parallel configurations. To tackle such scenarios, three-terminal equivalent networks like the wye (Y) (Figure 1 (a)) or tee (T) and delta (Δ) (Figure 1 (b)) or pi (π) networks come into play. These networks offer versatile solutions and are frequently encountered in various applications, including three-phase electrical systems, electrical filters, and matching networks.
1.0K
Resistance and Conductance01:25

Resistance and Conductance

A conductor's DC resistance at a given temperature is influenced by its resistivity, length, and cross-sectional area. Resistivity is an inherent property of the conductor material, with annealed copper serving as the international standard for measurement. For instance, the resistivity of hard-drawn aluminum at 20 degrees Celsius is 61% of the standard conductivity of annealed copper.
Various factors impact the resistance of a conductor. Spiraling in stranded conductors increases their...
534
Responses to Gravity and Touch02:26

Responses to Gravity and Touch

Gravitropism: Plant Responses to Gravity
42.1K