Related Experiment Video

Updated: Feb 16, 2026

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells
09:32

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Published on: February 8, 2018

15.6K

High-dimensional single-cell analysis predicts response to anti-PD-1 immunotherapy

Carsten Krieg1, Malgorzata Nowicka2,3, Silvia Guglietta4

  • 1Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland.

Nature Medicine
|January 9, 2018
PubMed

Insights

Predicting anti-PD-1 immunotherapy response in melanoma patients is crucial. High frequencies of specific monocytes (CD14+CD16-HLA-DRhi) before treatment strongly predict progression-free and overall survival in advanced melanoma.

Area of Science:

  • Immunology
  • Oncology
  • Biomarker Discovery

Background:

  • Immune-checkpoint inhibitors, particularly anti-programmed cell death protein 1 (PD-1) therapy, have transformed cancer treatment.
  • Despite successes in metastatic melanoma, many patients lack durable responses, necessitating predictive biomarkers.

Purpose of the Study:

  • To identify predictive biomarkers for anti-PD-1 immunotherapy response in stage IV melanoma patients.
  • To characterize immune cell subsets in peripheral blood before and during anti-PD-1 treatment.

Main Methods:

  • High-dimensional single-cell mass cytometry and bioinformatics analysis of peripheral blood immune cells.
  • Conventional flow cytometry for validation in an independent cohort.

Main Results:

  • While T cell responses were observed during therapy, pre-treatment frequencies of CD14+CD16-HLA-DRhi monocytes were significant predictors of progression-free and overall survival.
  • Validation confirmed the predictive power of these specific monocyte populations.

Conclusions:

  • The frequency of specific monocyte subsets in peripheral blood mononuclear cells (PBMCs) can serve as a predictive biomarker for anti-PD-1 immunotherapy outcomes in melanoma.
  • This finding may aid in clinical decision-making for immunotherapy selection.

More Related Videos

Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood06:07

Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood

Published on: February 5, 2020

6.2K
Identifying PD-1/PD-L1 Inhibitors with Surface Plasmon Resonance Technology07:04

Identifying PD-1/PD-L1 Inhibitors with Surface Plasmon Resonance Technology

Published on: May 2, 2025

1.2K

Related Concept Videos

Dimensional Analysis03:40

Dimensional Analysis

Dimensional analysis, also known as the factor label method, is a versatile approach for mathematical operations. The main principle behind this approach is: the units of quantities must be subjected to the same mathematical operations as their associated numbers. This method can be applied to computations ranging from simple unit conversions to more complex and multi-step calculations involving several different quantities and their units.
Conversion Factors and Dimensional Analysis
The unit...
65.3K
Dimensional Analysis01:27

Dimensional Analysis

Dimensional analysis is a valuable technique in fluid mechanics for simplifying complex problems by reducing them into dimensionless groups. These groups capture the essential relationships between the variables involved, allowing researchers and engineers to analyze fluid flow without dealing with each variable individually. This approach reduces the number of independent variables, allowing for easier analysis and better understanding of physical phenomena.
In fluid mechanics, dimensional...
697
Dimensional Analysis01:23

Dimensional Analysis

Dimensional analysis is a powerful tool that is used in physics and engineering to understand and predict the behavior of physical systems. The basic idea behind dimensional analysis is to express physical quantities in terms of fundamental dimensions such as the mass, length, and time. Derived dimensions like the velocity, acceleration, and force are derived from the combinations of these fundamental dimensions.
Dimensional analysis allows us to analyze and compare physical quantities on a...
2.2K
Dimensional Analysis02:19

Dimensional Analysis

The concept of dimension is important because every mathematical equation linking physical quantities must be dimensionally consistent, implying that mathematical equations must meet the following two rules. The first rule is that, in an equation, the expressions on each side of the equal sign must have the same dimensions. This is fairly intuitive since we can only add or subtract quantities of the same type (dimension). The second rule states that, in an equation, the arguments of any of the...
24.8K
Predicting Products: SN1 vs. SN202:27

Predicting Products: SN1 vs. SN2

Nucleophilic substitution reactions of alkyl halides can proceed via an SN1 or an SN2 mechanism. While in SN2 reactions, the nucleophile attacks the substrate simultaneously as the leaving group departs, in SN1 reactions, the substrate first dissociates to give the carbocation intermediate. Various factors such as the structure of the substrate, the strength of the nucleophile, and the nature of the solvent promote one mechanism over the other.
With increased substitution on the alkyl halide,...
17.4K
Tumor Immunotherapy01:27

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
2.0K