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Functional interrogation and mining of natively paired human VH:VL antibody repertoires

Bo Wang1, Brandon J DeKosky2,3,4, Morgan R Timm2

  • 1Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas, USA.

Nature Biotechnology
|January 9, 2018
PubMed

We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (VH:VL) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly neutralizing antibodies (bNAbs) from an HIV-1 slow progressor and high-affinity neutralizing antibodies against Ebola virus glycoprotein and influenza hemagglutinin.

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