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Updated: Feb 16, 2026

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L-tyrosine polymerization-based ultrasensitive multi-analyte enzymatic biosensor.

Lanjunzi Liu1, Xiuzhi Kang1, Chao Chen1

  • 1Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.

Talanta
|January 10, 2018
PubMed
Summary

A novel biosensor uses L-tyrosine polymerization to immobilize enzymes for detecting multiple analytes like BPA, glucose, and chromium. This multi-analyte biosensor offers high sensitivity and stability for diverse applications.

Keywords:
Bisphenol ACr(III)Cr(VI)Glucose biosensingOxidative polymerization of L-tyrosinePhenol

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Area of Science:

  • Biotechnology
  • Biosensor technology
  • Electrochemistry

Background:

  • Enzyme immobilization is crucial for biosensor development.
  • Developing multi-analyte detection systems remains a challenge.
  • Tyrosinase and glucose oxidase are key enzymes for various sensing applications.

Purpose of the Study:

  • To develop a novel amperometric bi-enzyme biosensor for simultaneous multi-analyte detection.
  • To utilize L-tyrosine polymerization for efficient enzyme immobilization.
  • To assess the biosensor's performance in detecting specific analytes and inhibitors.

Main Methods:

  • Development of a bi-enzyme biosensor using tyrosinase (Tyr) and glucose oxidase (GOx).
  • Immobilization of Tyr and GOx via L-tyrosine polymerization (PLT) on a gold electrode.
  • Amperometric detection of analytes including bisphenol A (BPA), phenol, Cr(III), glucose, and Cr(VI).

Main Results:

  • The PLT-Tyr-GOx/Au electrode effectively immobilized high-load, high-activity enzymes.
  • The biosensor demonstrated efficient sensing for both enzyme substrates (glucose, BPA, phenol) and inhibitors (Cr(VI), Cr(III)).
  • High sensitivity, precision, stability, and fabrication simplicity were achieved.

Conclusions:

  • A novel and efficient multi-analyte biosensor was successfully developed using L-tyrosine polymerization for enzyme immobilization.
  • The biosensor shows significant potential for diverse applications in biotechnology and environmental monitoring.
  • This approach offers a simple and effective method for creating stable and sensitive enzyme-based biosensors.