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Updated: Feb 16, 2026

Hydrogen Production and Utilization in a Membrane Reactor
Published on: March 10, 2023
Utilization of distillery wastewater for hydrogen production in one-stage and two-stage processes involving
Tatyana Laurinavichene1, Darya Tekucheva1, Kestutis Laurinavichius2
1Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.
Abstract:
In this study, distillery wastewater was treated by dark fermentation or photofermentation alone, and by sequential dark and photofermentation processes using anaerobic saccharolytic consortium and purple nonsulfur bacteria. Combination of dark and photofermentation resulted in the maximal H2 yield of 17.6L/L of distillery waste with chemical oxygen demand 40g/L. It is equivalent to 205kJ/L distillery wastewater and corresponds to recovery of approximately 4-8% of energy consumed during ethanol production. Optimal performance of photofermentation was observed at 20% concentration of pre-fermented distillery waste. In photofermentation, the range of the suitable distillery waste concentrations was extended and the H2 yield was improved by choosing the tolerant strain of purple bacteria Rhodobacter sphaeroides B-3059. After two stages, organic acids and sugars were completely consumed that means wastewater treatment concomitant to H2 production.
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