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Related Experiment Video

Updated: Feb 15, 2026

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Published on: November 17, 2009

Sampling the Mouse Hippocampal Dentate Gyrus.

Lisa Basler^1,2, Stephan Gerdes¹, David P Wolfer^1,3,4

¹Division of Functional Neuroanatomy, Institute of Anatomy, University of Zürich, Zürich, Switzerland.

Frontiers in Neuroanatomy

|January 10, 2018

Summary

The Gundersen-Jensen coefficient of error (CE) estimator accurately assesses sampling precision for neuroscience morphology. Optimal CE estimates for mouse dentate gyrus layers are achieved with a smoothness factor of 0, particularly in frontal sections.

Keywords:

C57BL/6 mice CE estimators Cavalieri estimator dentate gyrus stereology volume

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Published on: August 2, 2024

Area of Science:

Neuroscience
Quantitative Morphology
Stereology

Background:

Accurate quantitative morphological data in neurosciences relies on representative sampling.
Sampling variability can obscure significant group differences in statistical analyses.
Coefficient of error (CE) estimators assess if sampling precision is adequate for meaningful statistical outcomes.

Purpose of the Study:

To evaluate the performance of the Gundersen-Jensen CE estimator.
To determine optimal sampling parameters for the mouse hippocampal dentate gyrus.
To provide guidance on achieving desired sampling precision.

Main Methods:

Applied the Gundersen-Jensen CE estimator to mouse hippocampal dentate gyrus layers (molecular layer, granule cell layer, hilus).
Investigated the impact of smoothness factor (m=0 vs. m=1) on CE estimates.
Analyzed the effect of section orientation (frontal, horizontal, sagittal) on CE values.
Utilized 3D reconstructions and intense sampling for detailed CE analysis.

Main Results:

The Gundersen-Jensen CE estimator provides reliable estimates of sampling precision.
A smoothness factor (m) of 0 generally yielded better CE estimates for individual layers.
A smoothness factor (m) of 1 improved CE estimates for the combined dentate gyrus.
Frontal (coronal) sections were most efficient, yielding the smallest CE values for equivalent sampling effort.
Observed expected CE transitions with varying sampling intensity in 3D reconstructions.

Conclusions:

The Gundersen-Jensen CE estimator is a valuable tool for optimizing sampling strategies in neuroanatomy.
Smoothness factor and section orientation significantly influence CE estimates.
The study provides data to guide the selection of sampling intervals for desired precision in the mouse dentate gyrus.