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The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
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All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
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Neat1 in hematopoietic stem cells.

Noam Fallik1, Yael Bar-Lavan1,2,3, Yariv Greenshpan1,2

  • 1The Shraga Segal Department for Microbiology Immunology and Genetics, Faculty of Health Sciences, The Ben-Gurion University of the Negev, Be'er Sheva, Israel.

Oncotarget
|January 10, 2018
PubMed
Summary
This summary is machine-generated.

Neat1 long non-coding RNA and paraspeckles are expressed in hematopoietic stem cells (HSCs). Their expression decreases during differentiation into granulocytes, suggesting a role in hematopoiesis.

Keywords:
Neat1hematopoiesishematopoietic stem cellslong non-coding RNAsparaspeckles

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Area of Science:

  • Hematology
  • Molecular Biology
  • Genetics

Background:

  • Hematopoietic stem cells (HSCs) differentiate into diverse blood and immune cells.
  • Understanding HSC regulation is crucial for normal and malignant hematopoiesis.
  • Long non-coding RNAs (lncRNAs) roles in HSCs remain largely unexplored.

Purpose of the Study:

  • To investigate the expression and function of the lncRNA Neat1 in HSCs and during hematopoiesis.
  • To determine the presence and localization of paraspeckles in hematopoietic cells.

Main Methods:

  • RNA sequencing (RNA-seq) and RT-qPCR were used to detect Neat1 expression.
  • Immunofluorescence microscopy was employed to visualize Neat1 and paraspeckle proteins (NONO, PSPC1).
  • In vitro differentiation was induced to study Neat1 expression changes.

Main Results:

  • Neat1 is highly expressed in HSCs and progenitor cells, with reduced expression in granulocytes.
  • Neat1 forms distinct nuclear foci, and paraspeckle proteins NONO and PSPC1 also form nuclear aggregates.
  • Neat1 expression increases upon in vitro differentiation induction.

Conclusions:

  • This study provides the first evidence of Neat1 expression and paraspeckle formation in HSCs.
  • Neat1 and paraspeckles are present throughout hematopoiesis, with dynamic expression changes.
  • Neat1 may play a significant role in regulating HSC differentiation and hematopoiesis.