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Updated: Feb 15, 2026

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
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DNA damage in acute myeloid leukemia patients of Northern Mexico

Martha I Dávila-Rodríguez1, Elva I Cortés-Gutiérrez, Roberto Hernández-Valdés

  • 1Instituto Mexicano del Seguro Social. marthadavila@cibinmty.net.

European Journal of Histochemistry : EJH
|January 10, 2018
PubMed

Insights

DNA damage in acute myeloid leukemia (AML) patients was similar to controls, suggesting activated repair pathways. Inhibiting these pathways may improve chemotherapy effectiveness in AML treatment.

Area of Science:

  • Hematology
  • Molecular Biology
  • Genetics

Background:

  • Acute myeloid leukemia (AML) is a heterogeneous hematologic malignancy.
  • Understanding DNA damage and repair mechanisms in AML is crucial for treatment strategies.

Purpose of the Study:

  • To evaluate whole-genome DNA damage in peripheral blood leukocytes of AML patients versus controls.
  • To investigate the role of DNA repair pathways in newly diagnosed AML.

Main Methods:

  • Utilized DNA breakage detection-fluorescent in situ hybridization (DBD-FISH).
  • Compared DNA damage levels between AML patients and a healthy control group.

Main Results:

  • No significant difference in DNA damage was observed between AML patients and controls.
  • The similar DNA damage levels may indicate an upregulated DNA repair response in AML pathogenesis.

Conclusions:

  • The inherent DNA repair stimulation in AML might explain the lack of increased DNA damage.
  • Targeting and inhibiting these DNA repair pathways presents a potential strategy to enhance AML chemotherapy efficacy.
Keywords:
DNA breakage detection-fluorescence in situ hybridizationDNA damageMexico.acute myeloid leukemia

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