Related Experiment Video

Updated: Feb 15, 2026

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
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Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay

Published on: November 11, 2018

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Rab35 GTPase and cancer: Linking membrane trafficking to tumorigenesis

Ronan Shaughnessy1, Arnaud Echard1,2

  • 1Membrane Traffic and Cell Division Lab, Cell Biology and Infection Department, Institut Pasteur, Paris, France.

Traffic (Copenhagen, Denmark)
|January 10, 2018
PubMed

Insights

Rab35, a small GTPase, plays a dual role in cell biology. Activating mutations drive cancer, while its downregulation disrupts cell polarity and promotes migration, impacting cancer progression.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Rab35 is a small GTPase regulating critical cellular functions like membrane trafficking and cell polarity.
  • Emerging evidence highlights Rab35's oncogenic potential through activating mutations.
  • Rab35 downregulation is linked to inverted cell polarity and enhanced cell migration.

Purpose of the Study:

  • To review the known functions of Rab35 in cellular processes.
  • To discuss Rab35's role in membrane trafficking and signaling.
  • To explore the significance of Rab35 in cancer cell division and migration.

Main Methods:

  • Literature review of recent studies on Rab35.
  • Analysis of Rab35's involvement in membrane trafficking pathways.
  • Examination of Rab35's impact on cell polarity and migration.

Main Results:

  • Rab35 is implicated in diverse cellular activities including phagocytosis and cytokinesis.
  • Activating Rab35 mutations are associated with oncogenic properties.
  • Loss of Rab35 function inverts apico-basal polarity and promotes cell migration.

Conclusions:

  • Rab35 is a key regulator of membrane trafficking with significant implications in cancer.
  • Understanding Rab35-dependent pathways is crucial for deciphering cancer progression mechanisms.
  • Targeting Rab35 may offer therapeutic strategies for cancer treatment.
Keywords:
Rab proteinRab35cancercell migrationcell signalingcytokinesismembrane traffickingtumorigenesis

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