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Updated: Feb 15, 2026

Blood Collection from the American Horseshoe Crab, Limulus Polyphemus
Published on: October 13, 2008
THE FINE STRUCTURE OF THE AMEBOCYTE IN THE BLOOD OF LIMULUS POLYPHEMUS. I. MORPHOLOGY OF THE NORMAL CELL
Abstract:
The fine structure of the amebocyte (hemocyte) in the blood of Limulus polyphemus was reinvestigated regarding its normal state to resolve existing discrepancies in reporting before proceeding to studies of its participation in blood coagulation. The ovoid cell has all the organelles of a well equipped, metabolically active cell; having a nucleus, mitochondria, Golgi apparatus, endoplasmic reticulum, and ribosomes. However, no mitotic figures were seen. The most distinguishing characteristic of the cell is the presence of a population of large granules (predominant) and small, asymmetric dense granules (not always present). The large granules originate from the Golgi apparatus and the matrix of the developing granules undergoes a wide range of transitional patterns before assuming the mature dense condition. The transitional stages are interpreted as formative of new granules rather than stages of degranulation as previously reported. The small, asymmetric dense granules are considered to be a type of granule distinct from the larger more predominant type of granule.
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