Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Motility through Blebbing01:16

Cell Motility through Blebbing

2.6K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.6K
Microtubules in Cell Motility01:24

Microtubules in Cell Motility

4.8K
Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
4.8K
Imaging Studies III: Gastrointestinal Motility Studies and Virtual Colonoscopy01:26

Imaging Studies III: Gastrointestinal Motility Studies and Virtual Colonoscopy

451
This lesson explores three gastrointestinal imaging techniques: radionuclide testing, colonic transit studies, and virtual colonoscopy.
Radionuclide Testing
Radionuclide testing is a sophisticated medical technique for assessing gastrointestinal motility. It focuses on gastric emptying and colonic transit time. Radioactive markers track the movement of food through the digestive system, providing insights into gastrointestinal disorders.
In gastric emptying studies, a meal's liquid and...
451
Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

6.8K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
6.8K
Gastric Motility01:16

Gastric Motility

3.4K
Gastric motility is the coordinated contraction and relaxation of stomach muscles that convert ingested food into chyme, a semi-liquid substance ready for further digestion in the intestines. The process begins with the vagus nerve inducing the relaxation of the smooth muscles in the fundus and body of the stomach, allowing these regions to expand and accommodate up to approximately 1.5 liters of food and liquid.
Peristaltic Waves and Chyme Formation
Upon food entry, the stomach initiates...
3.4K
Gene-Environment Interactions01:20

Gene-Environment Interactions

1.3K
Gene expression is a dynamic process that is significantly influenced by environmental factors. This interaction underlies the complex nature of biological development and the phenotypic differences observed among individuals, even among those with identical genetic makeups. Factors such as radiation, temperature, behavior, nutrition, and stress play pivotal roles in determining how genes are expressed. The concept of the reaction range is central to understanding this interaction. It posits...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Essential nucleus-apical pole linkage maintains division fidelity during Plasmodium progeny formation.

The EMBO journal·2026
Same author

Stress granule phase separation in stress-responsive cytosolic extract-in-oil droplets.

Nature communications·2026
Same author

DNA origami-based virus-like particles: a new class of HIV vaccine candidates with superior immunogenic properties.

Signal transduction and targeted therapy·2026
Same author

RNA decay sharpens antigenic variation in trypanosomes.

Trends in parasitology·2026
Same author

A tissue-intrinsic mechanism sensitizes HIV-1 particles for TLR-triggered innate immune responses.

Nature communications·2026
Same author

Organelle mechanics: A brace for the Plasmodium basal body.

Current biology : CB·2026

Related Experiment Video

Updated: Feb 15, 2026

Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions
07:35

Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions

Published on: July 10, 2021

7.8K

Tailored environments to study motile cells and pathogens.

Julianne Mendi Muthinja1, Johanna Ripp1, Timothy Krüger2

  • 1Integrative Parasitology, Center for Infectious Diseases, Heidelberg University, Heidelberg, Germany.

Cellular Microbiology
|January 10, 2018
PubMed
Summary

Researchers developed new methods to study how cells and pathogens move in complex environments. Micropatterned pillar arrays help bridge the gap between 2D and 3D studies, revealing insights into cell migration and adaptation.

More Related Videos

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
10:13

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

9.6K
Author Spotlight: Studying Bacterial Growth in 3D Hydrogel Matrices
05:46

Author Spotlight: Studying Bacterial Growth in 3D Hydrogel Matrices

Published on: January 19, 2024

3.3K

Related Experiment Videos

Last Updated: Feb 15, 2026

Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions
07:35

Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions

Published on: July 10, 2021

7.8K
Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
10:13

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

9.6K
Author Spotlight: Studying Bacterial Growth in 3D Hydrogel Matrices
05:46

Author Spotlight: Studying Bacterial Growth in 3D Hydrogel Matrices

Published on: January 19, 2024

3.3K

Area of Science:

  • Cell Biology
  • Biophysics
  • Pathogen Motility

Background:

  • Cellular and pathogen migration is crucial in complex environments but often studied on simplified 2D surfaces.
  • Existing assays like 3D gels and microfluidics partially mimic natural settings.
  • A gap exists in understanding cellular behavior between 2D and 3D environments.

Purpose of the Study:

  • To introduce diverse assays that mimic complex cellular environments.
  • To focus on micropatterned pillar arrays as a method bridging 2D and 3D studies.
  • To investigate how environmental factors influence cell and pathogen motility and adaptation.

Main Methods:

  • Development of assays including substrates of defined elasticity, microfluidics, micropatterns, organotypic cultures, and 3D gels.
  • Utilizing polydimethylsiloxane (PDMS) to create micropatterned pillar arrays.
  • Applying these assays to study mechanoperception, gliding motility, swimming, and nuclear stability.

Main Results:

  • Micropatterned pillar arrays effectively bridge the gap between 2D and 3D cellular environment studies.
  • New insights were gained into mechanoperception in Caenorhabditis elegans.
  • Studies revealed mechanisms of gliding motility in Plasmodium and swimming in trypanosomes.
  • Nuclear stability in cancer cells was investigated in a more naturalistic context.

Conclusions:

  • Micropatterned pillar arrays offer a valuable tool for studying cell and pathogen behavior in complex, 3D-like environments.
  • These advanced assays enhance understanding of cellular and molecular adaptations to natural surroundings.
  • The findings contribute to a deeper comprehension of how environmental cues dictate cellular functions.