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Genetic background affects pathogenicity island function and pathogen emergence in Streptomyces.

Yucheng Zhang1, Guangde Jiang2, Yousong Ding2

  • 1Department of Plant Pathology, University of Florida, Gainesville, FL, 32611, USA.

Molecular Plant Pathology
|January 10, 2018
PubMed
Summary

Pathogenicity regions containing thaxtomin A (ThxA) can transfer between Streptomyces species. However, recipient strain genetics influence ThxA production and virulence, impacting new pathogen emergence.

Keywords:
Streptomycesbackgroundgeneticpathogenicity islandthaxtomin

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Area of Science:

  • Microbiology
  • Plant Pathology
  • Genetics

Background:

  • Thaxtomin A (ThxA) is a key pathogenicity determinant in Streptomyces species.
  • ThxA biosynthesis clusters are located on mobile pathogenicity islands (PAIs) like the toxicogenic region (TR) and PAIst.
  • The emergence of novel plant pathogens in Streptomyces is infrequent.

Purpose of the Study:

  • To investigate the widespread mobilization of ThxA-containing pathogenicity regions via mating.
  • To determine if TR and PAIst can confer plant pathogenicity to non-pathogenic strains.
  • To explore the potential of heterologous ThxA production as a natural herbicide.

Main Methods:

  • Conjugation experiments were performed to transfer ThxA biosynthetic clusters from S. scabiei and S. turgidiscabies into non-pathogenic Streptomyces species.
  • Transconjugants were analyzed for ThxA production and virulence phenotype.

Main Results:

  • ThxA biosynthetic clusters were successfully transferred into five non-pathogenic Streptomyces species.
  • Not all transconjugants produced ThxA or exhibited virulence, indicating recipient strain genetic background influences functionality.
  • Demonstrated potential for heterologous production of ThxA in non-pathogenic strains.

Conclusions:

  • Mobilization of ThxA-containing pathogenicity islands can occur between Streptomyces species.
  • The genetic background of recipient strains is crucial for the expression of ThxA and pathogenicity.
  • Heterologous production of ThxA offers potential for developing biodegradable herbicides.