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Updated: Aug 10, 2026

Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices
Published on: October 29, 2012
Muscarine-binding sites localized to cortical dopamine terminals
Abstract:
The effects of lesions to the mesocortical dopaminergic system on D2 dopamine receptors and muscarine receptors in the frontal cortex of the rat was examined. Four weeks following 6-hydroxydopamine lesioning of the ventral tegmental area, there was a 26% increase in the number of [3H]spiroperidol sites, and a 13% decrease in the number of [3H]oxotremorine-M sites in the frontal cortex, indicating a development of D2 receptor supersensitivity, as a result of deafferentation, and a loss of acetylcholine sites, as result of terminal degeneration. This demonstrates that in the frontal cortex, as in the striatum and nucleus accumbens, the activity of dopaminergic terminals may be partially modulated by cholinergic inputs.
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