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Updated: Feb 15, 2026

In Vivo 2-Photon Calcium Imaging in Layer 2/3 of Mice
Published on: March 13, 2008
Single layer molybdenum disulfide as an optical nanoprobe for 2 photon luminescence and second harmonic generation
Hong Zhong1, Lin Lu1, Yuanhao Wang1
1Department of Building Services Engineering, Hong Kong Polytechnic University, Hong Kong, China.
Abstract:
More recently, tremendous progress has been achieved in the development of two-dimensional semiconductor materials applied in catalyst, energy application, sensor device and bioengineering since the birth of graphene isolated from graphite. Layered molybdenum disulfide (MoS2 ) as an indirect gap semiconductor can efficiently emit photoluminescence (PL) excited by visible light, which shows a great potential in adaptive biological imaging. However, 1 photon PL of MoS2 for cell imaging purposes suffers from strong autofluorescence and ion-induced PL quenching. Herein, we report single layer small chitosan decorated MoS2 nanosheets as a nonbleaching, nonblinking optical nanoprobe under near infrared femtosecond laser excitation and their applications for strong 2 photon luminescence (TPL) and strong second harmonic generation (SHG) bioimaging. Furthermore, the TPL can resist the ion-induced quenching on the cellular membrane. The proposed TPL and SHG of single-layer MoS2 show great potential for real-time, deep, multiphoton and three-dimensional bioimaging under low-power laser excitation.
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