Related Experiment Video
Updated: Feb 15, 2026

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
Published on: January 3, 2015
Targeting the Expression of Cathepsin B Using CRISPR/Cas9 System in Mammalian Cancer Cells
Manu Gnanamony1, Christopher S Gondi2,3,4
1Department of Pediatrics, University of Illinois College of Medicine at Peoria, Peoria, IL, USA.
Abstract:
Cathepsin B belongs to a family of cathepsins and plays an important role in normal physiological functions in the cell. However, overexpression of cathepsin B has been associated with different malignancies, and this has made it an attractive pharmacological target. The advent of CRISPR-Cas9 technology has allowed researchers to efficiently knock down genes with very less nonspecific activity compared to earlier methods. The protocol described below will enable investigators to develop cathepsin B knockdown stable cells and explains ways to study the knockdown.
Related Concept Videos
CRISPR
CRISPR and crRNAs
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR/Cas9 Genome Editing
Targeted Cancer Therapies
There are several types of targeted therapies against...
Cell Specific Gene Expression
Cell Specific Gene Expression

