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[Swine serum increases hybridoma stability].

Iu A Mantsygin, N V Sviatukhina

    Tsitologiia
    |July 1, 1985
    PubMed
    Summary

    Swine serum enhances hybridoma cell line stability and antibody production compared to fetal calf serum. Hybridomas cultured in swine serum maintained antibody production for 9 months, unlike those in fetal calf serum.

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    Area of Science:

    • Immunology
    • Biotechnology
    • Cell Biology

    Background:

    • Hybridoma technology is crucial for monoclonal antibody production.
    • Cell culture media composition significantly impacts hybridoma performance and antibody yield.
    • Fetal calf serum (FCS) and swine serum (SS) are common supplements in hybridoma culture media.

    Purpose of the Study:

    • To compare the effects of fetal calf serum and swine serum on the stability and antibody production of lambda phage-specific monoclonal antibody-producing hybridoma cell lines.
    • To determine the optimal serum supplement for long-term hybridoma culture and consistent antibody expression.

    Main Methods:

    • Three hybridoma cell lines were cultured for 9 months.
    • Cultures were supplemented with either 10% fetal calf serum or 5% swine serum.
    • Proliferation rates, maximum population densities, antibody production stability, and antibody activity were monitored.

    Main Results:

    • All hybridoma clones exhibited similar proliferation rates and maximum population densities in both media.
    • Hybridomas cultured in swine serum maintained antibody production throughout the 9-month experiment.
    • One clone in swine serum showed a 10^3-10^4 fold increase in antibody activity.
    • Two of three clones in fetal calf serum lost antibody production within 3-15 weeks.

    Conclusions:

    • Swine serum significantly enhances the stability and sustained antibody production of hybridoma cell lines compared to fetal calf serum.
    • Swine serum appears to be a superior supplement for long-term hybridoma cultures requiring consistent monoclonal antibody expression.
    • These findings have implications for optimizing hybridoma culture protocols in biotechnology and research settings.

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