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Updated: Feb 15, 2026

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
Published on: November 1, 2018
Rapid detection of a dengue virus RNA sequence with single molecule sensitivity using tandem toehold-mediated
Mingxuan Gao1, Douglas Daniel, Hongyan Zou
1Biodesign Center for BioEnergetics, Arizona State University, Tempe 85287, USA. Shengxi.Chen.1@asu.edu Sid.Hecht@asu.edu.
Abstract:
A DNA tetrahedron having a single-strand DNA edge was used to detect a dengue virus RNA sequence. Novel tandem toehold-mediated displacement reactions (tTMDR) were developed to amplify the fluorescence signal from the DNA tetrahedron. Using an excess of the DNA tetrahedron each target RNA was recycled about 103 times during the tTMDR process. This amplification process was used for the sensitive detection of dengue virus RNA in this study. As few as 6 copies of RNA per sample could be detected using a photon count technique with single molecule sensitivity.
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