Effects of PrP^C on DF-1 cells' biological processes and RNA-seq-based analysis of differential genes

To reveal the effects of PrP^C on cells' biological processes and on gene expression. We established stable DF-1 (PrP^C -knockdown (KD)) cells, and combined with DF-1 (wt) and DF-1 (PrP^C -overexpression (OE)) cells that we previously established we studied the effects of chicken PrP^C (PrP^C ) on DF-1 cells' processes. Then by using high throughput sequencing technology (HTS) and bioinformatics, we analyzed the differentially expressed genes (DEGs) between these cells. The results show that compared with DF-1 (wt) and DF-1 (PrP^C -scramble), DF-1 (PrP^C -KD) are significantly decreased in adhesion, proliferation, formation rate of colony and cells number of colony, scratch wound healing rate, cells number of invasion and migration, S phase cell populations, but the apoptosis rate and G1 phase cell populations are significantly increased. Conversely, all of these features in DF-1 (PrP^C -OE) are opposite. In addition, compared with DF-1 (wt), we found that there are totally 1055 DE genes between DF-1 (PrP^C -KD) and DF-1 (PrP^C -OE) cells. After Go and pathway enrichment analysis, we know that these DEGs are significantly enriched in cell, cell part, cellular process, and metabolic pathway. In short, we found that PrP^C can promote DF-1 cells' processes except apoptosis. Furthermore, PrP^C involves in the focal adhesion, cancer, ribosome, metabolic pathways, and so forth, and the overexpression of PrP^C can promote the pathway of amoebiasis, but its down-regulation can promote the pathway of serotonergic synapse. However, the details are keeping unknown and that would be our next research.