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Updated: Feb 15, 2026

Thin-layer Chromatographic TLC Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
Published on: March 27, 2014
Characterisation of Antimicrobial Properties of Extracts of Selected Medicinal Plants
Monika Cioch1, Paweł Satora1, Magdalena Skotniczny1
1Department of Fermentation Technology and Technical Microbiology, Faculty of Food Technology, University of Agriculture, Kraków, Poland.
Abstract:
The scope of the experiments included analysis of the antimicrobial activity of ethanolic, methanolic and aqueous extracts against bacterial and fungal cultures and determination of the minimum inhibitory concentration of plant extracts tested microbial growth. Analysis of the antifungal and antibacterial activity was carried out by the disc diffusion method using paper discs. In the experiment 11 species of microorganisms - 8 bacterial and 3 fungal strains were used. The highest antimicrobial activity against the tested strains was demonstrated by black elder (Sambucus nigra L.), black locust (Robinia pseudoacacia L.) and lingonberry (Vaccinium vitis-idaea L.) extracts. The study showed the diverse morphological activity of specific parts of elderberry and quince, which is the effect of different polyphenolic profile of these plants. The yeast Saccharomyces cerevisiae, Escherichia coli, Pseudomonas putida and Bacillus subtilis showed the highest sensitivity to the effect of extracts of the analysed plants. As a positive control three antibiotics - amphotericin B, vancomycin and amoxicillin with clavulanic acid were used.
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