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Updated: Feb 15, 2026

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
Published on: May 2, 2018
Analyses of Plasmids Harbouring Quinolone Resistance Determinants in Enterobacteriaceae Members
Bayri Erac1, Fethiye Ferda Yilmaz1, Ismail Ozturk1
1Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Ege University, Izmir, Turkey.
Abstract:
The aim of this study was to explore the plasmid characteristics of eight clinical Enterobacteriaceae strains containing extended broad spectrum beta-lactamases and plasmid-mediated quinolone resistance. Plasmids were transferred by conjugation or transformation and resistance determinants were investigated by PCR. We showed that at least one plasmid harbouring qnrB or qnrS determinant was transferred by conjugation in five isolates. QepA determinant was confirmed to be on a non-conjugative plasmid. We found at least one beta-lactamase gene in seven of the eight clinical isolates having plasmid-mediated quinolone resistance, which indicated that these two resistance determinants were mostly on the same conjugative plasmids.
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