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Related Experiment Video

Updated: Feb 15, 2026

Use of Alu Element Containing Minigenes to Analyze Circular RNAs
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Facilitating circular permutation using Restriction Free (RF) cloning.

Boudhayan Bandyopadhyay1, Yoav Peleg2

  • 1Department of Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel.

Protein Engineering, Design & Selection : PEDS
|January 11, 2018
PubMed
Summary
This summary is machine-generated.

Circular permutation is a key technique for studying protein topology, folding, and function. A new Restriction Free (RF) method offers a faster, more cost-effective way to create circular protein variants.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Circular permutation is crucial for investigating the impact of topology on protein folding and function.
  • Traditional methods for generating circular protein variants rely on restriction enzyme-based gene cloning, which can be time-consuming and expensive.

Purpose of the Study:

  • To introduce a novel and efficient method for creating circular protein variants.
  • To provide a faster and more cost-effective alternative to existing techniques for protein circular permutation.

Main Methods:

  • Development of a Restriction Free (RF) cloning strategy for generating circular permutations.
  • Application of the RF approach to create circular protein variants.

Main Results:

  • The Restriction Free (RF) method enables the rapid generation of circular protein constructs.
  • This new approach is more cost-effective compared to traditional restriction enzyme-based cloning methods.

Conclusions:

  • The Restriction Free (RF) method presents a significant advancement in protein engineering.
  • This technique facilitates the study of protein topology, folding, and function through efficient generation of circular variants.