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Related Experiment Video

Updated: Feb 15, 2026

The Slice Culture Method for Following Development of Tooth Germs In Explant Culture

The Slice Culture Method for Following Development of Tooth Germs In Explant Culture

Published on: November 13, 2013

Esrrb Complementation Rescues Development of Nanog-Null Germ Cells.

Man Zhang¹, Harry G Leitch², Walfred W C Tang³

¹MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, Scotland.

|January 11, 2018

Summary

The transcription factor Nanog is crucial for germ cell development. However, Esrrb can functionally replace Nanog in germ cells, restoring PGC numbers and fertility in mice.

Keywords:

PGCLCs competence naive pluripotency primordial germ cells transcription factors

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Last Updated: Feb 15, 2026

The Slice Culture Method for Following Development of Tooth Germs In Explant Culture

The Slice Culture Method for Following Development of Tooth Germs In Explant Culture

Published on: November 13, 2013

Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture Devices

Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture Devices

Published on: August 14, 2015

Depletion of Specific Cell Populations by Complement Depletion

Depletion of Specific Cell Populations by Complement Depletion

Published on: February 5, 2010

Area of Science:

Developmental Biology
Stem Cell Biology
Genetics

Background:

Nanog and Esrrb are key transcription factors in embryonic stem cells (ESCs) and primordial germ cells (PGCs).
Esrrb can functionally substitute for Nanog in ESCs, but its role in germline development is unclear.

Purpose of the Study:

To investigate whether Esrrb can functionally replace Nanog during germ cell development in vivo and in vitro.
To determine the impact of Nanog deletion on PGC development and the potential of Esrrb to rescue these defects.

Main Methods:

Germline deletion of Nanog in mice.
Analysis of PGC numbers and development at midgestation.
In vitro differentiation of PGC-like cells (PGCLCs) from Nanog-null ESCs.
Induction of Esrrb expression in Nanog-null PGCLCs.
Knockin of Esrrb into the Nanog locus in vivo.

Main Results:

Germline deletion of Nanog caused a 5-fold reduction in PGC numbers but did not prevent germline development.
Nanog-null ESCs showed impaired PGCLC differentiation with reduced proliferation and increased apoptosis.
Induced Esrrb expression rescued PGCLC numbers similarly to Nanog.
Esrrb knockin into the Nanog locus restored PGC numbers to wild-type levels and resulted in fertile mice.

Conclusions:

Esrrb can functionally replace Nanog in germ cells, rescuing PGC development and restoring fertility.
This study demonstrates the potential of Esrrb as a substitute for Nanog in germline development.