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3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
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Long Noncoding RNA ATB Promotes Proliferation, Migration, and Invasion in Bladder Cancer by Suppressing MicroRNA-126

Xingquan Zhai1, Wei Xu1

  • 1Department of Urology, Zoucheng People's Hospital, Zoucheng, Shandong, P.R. China.

Oncology Research
|January 12, 2018
PubMed

Insights

Long noncoding RNA activated by TGF-β (lncRNA-ATB) promotes bladder cancer progression by downregulating miR-126 and upregulating KRAS. This suggests lncRNA-ATB is a potential therapeutic target for bladder cancer.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Bladder cancer remains a significant health concern with limited therapeutic options.
  • Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development.
  • The specific functions of lncRNA-ATB in bladder cancer are not fully understood.

Purpose of the Study:

  • To investigate the biological functions of lncRNA-ATB in bladder cancer cells.
  • To elucidate the molecular mechanisms underlying lncRNA-ATB's role in bladder cancer.
  • To assess the potential of lncRNA-ATB as a prognostic biomarker and therapeutic target.

Main Methods:

  • T24 bladder cancer cells were transfected with vectors/shRNA or mimic/inhibitor for lncRNA-ATB, miR-126, and KRAS.
  • Cell viability, migration, invasion, and apoptosis assays were performed.
  • Protein levels of apoptosis-related factors and signaling pathways (PI3K/AKT, mTOR) were measured.
  • Dual-Luciferase Reporter assay was used to confirm molecular interactions.

Main Results:

  • Overexpression of lncRNA-ATB significantly enhanced cell viability, migration, and invasion.
  • lncRNA-ATB acts as a molecular sponge for miR-126, leading to its downregulation.
  • miR-126 directly targets KRAS, negatively regulating its expression.
  • KRAS overexpression promoted cell viability, migration, invasion, and activated PI3K/AKT/mTOR pathways.

Conclusions:

  • lncRNA-ATB functions as an oncogene in bladder cancer by promoting proliferation, migration, and invasion.
  • The lncRNA-ATB/miR-126/KRAS axis plays a crucial role in bladder cancer progression.
  • lncRNA-ATB represents a potential prognostic biomarker and therapeutic target for bladder cancer.

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