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Updated: Feb 15, 2026

Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae
Published on: December 29, 2017
Analysis of Histone Modifications in Acute Myeloid Leukaemia Using Chromatin Immunoprecipitation
Benjamin J Shields1, Andrew Keniry2,3, Marnie E Blewitt2,3
1Australian Centre for Blood Diseases, Monash University, Melbourne, VIC, Australia.
Abstract:
Chromatin Immunoprecipitation (ChIP) using antibodies specific for histone modifications is a powerful technique for assessing the epigenetic states of cell populations by either quantitative PCR (ChIP-PCR) or next generation sequencing analysis (ChIP-Seq). Here we describe the procedure for ChIP of histone marks in myeloid leukaemia cell lines and the subsequent purification of genomic DNA associated with repressive and activating histone modifications for further analysis. This procedure can be widely applied to a variety of histone marks to assess both activating and repressive modifications in the context of myeloid leukaemia.
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