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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Short-distance Transport of Resources

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Short-distance transport refers to transport that occurs over a distance of just 2-3 cells, crossing the plasma membrane in the process. Small uncharged molecules, such as oxygen, carbon dioxide, and water, can diffuse across the plasma membrane on their own. In contrast, ions and larger molecules require the assistance of transport proteins due to their charge or size. Transport across membranes also occurs within individual cells, playing a variety of essential roles for the plant as a whole.
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Xylem and Transpiration-driven Transport of Resources02:03

Xylem and Transpiration-driven Transport of Resources

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The xylem of vascular plants distributes water and dissolved minerals that are taken up by the roots to the rest of the plant. The cells that transport xylem sap are dead upon maturity, and the movement of xylem sap is a passive process.
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Exercise and Muscle Performance01:27

Exercise and Muscle Performance

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Exercise induces a range of adaptations in muscle tissue, depending on the type and duration of activity. Such physical training can be broadly categorized into two types: endurance exercises and resistance exercises.
Endurance exercises
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Pathophysiology of Cardiac Performance

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Typical heart performance is influenced by heart rate, rhythm, myocardial contraction, and metabolism or blood flow. The cardiac muscle exhibits distinct electrophysiological features, including pacemaker activity and calcium channel control, which play a vital role in the heart's response to various drugs. The autonomic nervous system, comprising the sympathetic and parasympathetic branches, regulates heart rate. Sympathetic activation increases heart rate, while parasympathetic activation...
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Related Experiment Video

Updated: Feb 15, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
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A Comprehensive Protocol Resource for Performing Pooled shRNA and CRISPR Screens.

Leonie A Cluse1, Iva Nikolic2, Deborah Knight1

  • 1Gene Regulation Laboratory, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|January 12, 2018
PubMed
Summary

This guide provides comprehensive protocols for pooled shRNA and CRISPR screening, enabling researchers to identify gene targets and analyze results for any laboratory screening project.

Keywords:
Drop out screenLentivirusPooled CRISPRPooled shRNA

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Area of Science:

  • Molecular Biology
  • Genetics
  • Bioinformatics

Background:

  • Pooled shRNA and CRISPR screening are powerful tools for functional genomics.
  • Implementing these screening technologies can be complex and resource-intensive.
  • A standardized, comprehensive approach is needed to facilitate their adoption.

Purpose of the Study:

  • To provide a complete set of protocols for conducting pooled shRNA and/or CRISPR screening.
  • To guide researchers through the entire screening process, from library creation to data analysis.
  • To make pooled screening technologies accessible to a wider range of laboratories.

Main Methods:

  • Detailed protocols for identifying and validating positive controls and hits.
  • Methods for establishing viral titers in various cell lines.
  • Strategies for library design, screening, sequencing, and bioinformatics analysis.

Main Results:

  • A comprehensive resource covering all stages of a pooled screening project.
  • Enables successful implementation of shRNA and CRISPR screening.
  • Facilitates the identification of functional genetic elements.

Conclusions:

  • This compendium offers a standardized framework for pooled screening.
  • Empowers researchers to conduct complex genetic screens.
  • Broadens the accessibility of high-throughput screening methodologies in diverse research settings.