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Updated: Feb 15, 2026

Implementation of a Coherent Anti-Stokes Raman Scattering CARS System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope
Published on: July 17, 2016
Two-frequency CARS imaging by switching fiber laser excitation
Eric C Rentchler1, Ruxin Xie2, Rongqing Hui2
1Department of Chemistry, University of Kansas, Lawrence, Kansas, 66045.
Abstract:
To fully exploit the power of coherent Raman imaging, techniques are needed to image more than one vibrational frequency simultaneously. We describe a method for switching between two vibrational frequencies based on a single fiber-laser source. Stokes pulses were generated by soliton self-frequency shifting in a photonic crystal fiber. Pump and Stokes pulses were stretched to enhance vibrational resolution by spectral focusing. Stokes pulses were switched between two wavelengths on the millisecond time scale by a liquid-crystal retarder. Proof-of-principle is demonstrated by coherent anti-Stokes Raman imaging of polystyrene beads embedded in a poly(methyl methacrylate) (PMMA) matrix. The Stokes shift was switched between 3,050 cm-1 , where polystyrene has a Raman transition, and 2,950 cm-1 , where both polystyrene and PMMA have Raman resonances. The method can be extended to multiple vibrational modes.
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