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Buffalopox.

Sergey V Borisevich, S S Marennikova, L F Stovba

    Voprosy Virusologii
    |January 12, 2018
    PubMed
    Summary
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    Buffalopox is a contagious viral disease in buffaloes with zoonotic potential, causing significant economic losses. PCR detection of the C18L gene aids in differentiating buffalopox virus from other orthopoxviruses.

    Area of Science:

    • Veterinary Virology
    • Zoonotic Diseases
    • Molecular Diagnostics

    Background:

    • Buffalopox is a contagious viral disease affecting milch buffaloes and occasionally cows, with significant zoonotic implications for humans, particularly milkers.
    • The disease presents with high morbidity (80%) and characteristic lesions on the udder, teats, and other body parts, leading to economic losses due to reduced productivity.

    Purpose of the Study:

    • To investigate the molecular characteristics of buffalopox virus for specific detection and differentiation from other orthopoxviruses.
    • To understand the epidemiological and molecular aspects of buffalopox for effective disease control.

    Main Methods:

    • Polymerase Chain Reaction (PCR) was employed for the specific detection of buffalopox virus.
    • Analysis focused on the C18L gene, which encodes a host range-determining ankyrin repeat protein.

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    Main Results:

    • The C18L gene's open reading frame length varies among orthopoxviruses (150 nucleotides in buffalopox virus vs. 453-759 nucleotides in related viruses).
    • This genetic variation allows for specific detection and differentiation of buffalopox virus using PCR targeting the C18L gene.

    Conclusions:

    • The C18L gene provides a reliable target for specific buffalopox virus detection and differentiation from other orthopoxviruses.
    • Further systematic studies on buffalopox epidemiology, reservoirs, transmission, and molecular organization are crucial for developing effective diagnostic and prophylactic measures.