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Atrial natriuretic factor in human blood
The Journal of Clinical Investigation
|October 1, 1985
Summary
Atrial natriuretic factor (ANF) is confirmed as a circulating hormone in men. Plasma ANF levels significantly increase following isotonic saline infusion, indicating secretion in response to volume expansion.
Area of Science:
- Endocrinology
- Cardiovascular Physiology
Background:
- Atrial natriuretic factor (ANF) is a peptide hormone with known cardiovascular and renal effects.
- Its presence and role as a circulating hormone in humans, particularly in response to volume changes, require further elucidation.
Purpose of the Study:
- To develop and validate a radioimmunoassay for quantifying ANF in human plasma.
- To characterize the molecular nature of circulating ANF in humans.
- To investigate the effect of isotonic volume expansion on plasma ANF levels.
Main Methods:
- Development of a radioimmunoassay for human plasma ANF using affinity chromatography for extraction.
- Analysis of plasma and atrial tissue ANF using SDS-PAGE and reversed-phase HPLC.
- Measurement of plasma ANF levels before and after saline infusion in healthy subjects.
Main Results:
- A specific radioimmunoassay for plasma ANF was established.
- Human plasma ANF primarily exists as a 3,000-mol-wt form, distinct from multiple forms found in atrial tissue.
- Plasma ANF levels significantly increased after isotonic saline infusion, confirming ANF's response to volume expansion.
Conclusions:
- Atrial natriuretic factor (ANF) is a circulating hormone in men.
- Plasma ANF levels are elevated in response to isotonic volume expansion.
- These findings support ANF's role in fluid and electrolyte balance regulation.
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