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Related Concept Videos

Quality Control01:05

Quality Control

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Quality control is one of the three cyclical quality assurance activities that help keep a system under statistical control. Typical quality control activities include creating quality control charts, conducting proficiency testing, and documenting and archiving results.
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Mass Spectrometry: Overview01:19

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule; a molecule with an odd number of nitrogen atoms produces a molecular ion with an odd molecular weight. Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit strong molecular ion peaks, but acyclic...
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Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
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The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
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QCloud: A cloud-based quality control system for mass spectrometry-based proteomics laboratories.

Cristina Chiva1,2, Roger Olivella1,2, Eva Borràs1,2

  • 1Proteomics Unit, Centre de Regulació Genòmica (CRG), Barcelona Institute of Science and Technology (BIST), Barcelona, Barcelona.

Plos One
|January 12, 2018
PubMed
Summary

QCloud is a new cloud-based system for automated quality assessment in mass spectrometry-based proteomics. It helps labs ensure high-quality data, improve inter-laboratory comparisons, and evaluate instrument performance.

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Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Bioinformatics

Background:

  • Mass spectrometry-based proteomics is vital for biomedical research but faces analytical challenges.
  • Automated quality control is needed to improve data quality and inter-laboratory comparisons.

Purpose of the Study:

  • To introduce QCloud, a cloud-based system for automated quality assessment in proteomics.
  • To provide a user-friendly, accessible tool for daily instrument evaluation and data archiving.

Main Methods:

  • Developed a cloud-based system (QCloud) with a user-friendly interface.
  • Implemented automated data processing, archiving, and unbiased instrument evaluation.
  • Ensured compatibility with common targeted and untargeted proteomics workflows and various data formats.

Main Results:

  • QCloud successfully supports daily quality assessment for proteomics laboratories.
  • The system facilitates automated data processing, archiving, and reporting of incidences.
  • QCloud is open-source and available to the scientific community.

Conclusions:

  • QCloud addresses the need for automated quality control in proteomics.
  • The system enhances data quality, reproducibility, and instrument performance assessment.
  • QCloud promotes wider adoption of quality control practices in proteomics research.