Total fractionation and characterization of the water-soluble polysaccharides isolated from Enteromorpha intestinalis

Water-soluble crude polysaccharides (WE) were extracted from the Enteromorpha intestinalis with hot water and further fractionated on a DEAE-52 Cellulose chromatography column and Sepharose CL-6B gel-permeation chromatography to afford one neutral fraction (WE-11) and five acidic fractions (WE-21, WE-31, WE-32, WE-41 and WE-42). Monosaccharide analysis showed that WE-32, WE-41 and WE-42 were all composed of Rha and GlcA, WE-21 and WE-31 contained Man and Rha, and WE-11 was composed of GlcA and Xyl. The molecular weight of WE-11, WE-21, WE-31 WE-32, WE-41 and WE-42 were 2.23 × 10⁵, 1.42 × 10⁵, 1.42 × 10⁵, 2.62 × 10⁴, 2.23 × 10⁵ and 8.09 × 10^4, respectively. Then, antioxidant activities in vitro were investigated on the basis of DPPH radical assay, reducing power assay, hydroxyl radical assay and superoxide radical assay. Of all polysaccharides fractions, WE-32 and WE-41 had relative higher content of sulfate and GlcA. In addition, WE-32 and WE-41 showed relative stronger antioxidant activity and inhibitory activity in vitro. The antioxidant activities of polysaccharides were not a function of a single factor but a combination of several factors, such as monosaccharide composition, molecular weights, protein content, uronic acid and sulfate content.