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Updated: Feb 15, 2026

Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia
Published on: November 12, 2011
Mutant Huntingtin Secretion in Neuro2A Cells and Rat Primary Cortical Neurons
Katarina Trajkovic1, Hyunkyung Jeong1, Dimitri Krainc1
1Department of Neurology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.
Abstract:
Quantitative analysis of proteins secreted from the cells poses a challenge due to their low abundance and the interfering presence of a large amount of bovine serum albumin (BSA) in the cell culture media. We established assays for detection of mutant huntingtin (mHtt) secreted from Neuro2A cell line stably expressing mHtt and rat primary cortical neurons by Western blotting. Our protocol is based on reducing the amounts of BSA in the media while maintaining cell viability and secretory potential, and concentrating the media prior to analysis by means of ultrafiltration.
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