Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Base Pairing02:27

DNA Base Pairing

33.8K
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
33.8K
DNA Base Pairing02:27

DNA Base Pairing

33.0K
33.0K
DNA Helicases00:55

DNA Helicases

24.2K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
24.2K
DNA-only Transposons02:57

DNA-only Transposons

17.6K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
17.6K
From DNA to Protein03:06

From DNA to Protein

22.6K
The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
22.6K
DNA Isolation01:34

DNA Isolation

200.3K
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
200.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Changes in physical activity behavior in children and adolescents: a systematic review and meta-analysis based on a trans-theoretical model.

European review for medical and pharmacological sciences·2022
Same author

Nontyphoidal salmonella urinary tract infection in a case of hyperparathyroidism and nephrocalcinosis.

The West Indian medical journal·2014
Same author

Intrahepatic IFN-alpha expression in liver specimens from HBV-infected patients with different outcomes.

European review for medical and pharmacological sciences·2013
Same author

A boy with cleft palate, hearing impairment, microcephaly, micrognathia and psychomotor retardation and a microdeletion in 6p25.3 involving the DUSP22 gene.

Genetic counseling (Geneva, Switzerland)·2013
Same author

Sex-specific differences in the relationship between the single-nucleotide polymorphism rs2298804 of FCER1A and the susceptibility to systemic lupus erythematosus in a Chinese Han population.

Clinical and experimental dermatology·2013
Same author

Partial trisomy 1q (1q42.13-->qter) and partial monosomy 6q (6q27-->qter) in a girl with single median maxillary central incisor, corpus callosum dysgenesis and developmental delay.

Genetic counseling (Geneva, Switzerland)·2013

Related Experiment Video

Updated: Feb 15, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

106.5K

DNA methylation assay using droplet-based DNA melting curve analysis.

F-W Liu1, H-F Liao, S-P Lin

  • 1Dept. of Bio-Industrial Mechatronics Engineering, National Taiwan University, Taipei, Taiwan, ROC. yenwenlu@ntu.edu.tw.

Lab on a Chip
|January 13, 2018
PubMed
Summary

This study introduces a novel microchip for detecting DNA methylation. The platform offers a robust, inexpensive method for quantifying epigenetic changes, crucial for understanding disease associations.

More Related Videos

Methylated DNA Immunoprecipitation
21:24

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

24.2K
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

3.1K

Related Experiment Videos

Last Updated: Feb 15, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

106.5K
Methylated DNA Immunoprecipitation
21:24

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

24.2K
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

3.1K

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Biomedical Engineering

Background:

  • DNA methylation is a key epigenetic regulator of gene expression, implicated in various diseases.
  • Accurate and affordable methods for detecting DNA methylation status in specific genomic regions are needed.

Purpose of the Study:

  • To develop and validate an on-chip analytical technique for cytosine methylation detection using microfluidic droplets.
  • To enable robust and inexpensive quantification of DNA methylation levels.

Main Methods:

  • Genomic DNA samples were encapsulated in droplets within a microchannel.
  • A temperature gradient (60-85 °C) induced DNA denaturation, generating melting curves.
  • Droplets acted as discrete reactors for liquid-phase DNA melting curve analysis.

Main Results:

  • The microchip demonstrated enhanced discrimination ability for different DNA methylation percentages due to high heating rates and thermal stability.
  • The platform showed lower volume consumption compared to commercial qPCR machines.
  • Quantification of Oct-4 methylation levels in the distal enhancer region was achieved post-bisulfite treatment and asymmetric PCR.

Conclusions:

  • The proposed microchip offers a sensitive, cost-effective, and efficient platform for DNA methylation analysis.
  • This technology has potential applications in disease research and diagnostics requiring precise epigenetic profiling.