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Updated: Feb 15, 2026

Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp
Published on: November 6, 2021
Cryobiotechnology of apple (Malus spp.): development, progress and future prospects.
Min-Rui Wang1, Long Chen1, Jaime A Teixeira da Silva2
1State Key Laboratory of Crop Stress Biology in Arid Region, College of Horticulture, Northwest Agriculture & Forestry University, Yangling, 712100, Shaanxi, People's Republic of China.
Cryopreservation preserves valuable apple (Malus spp.) genes for breeding elite cultivars. Cryotherapy enhances virus-free plant production, supporting sustainable apple industry development.
Area of Science:
- Horticultural Science
- Plant Biotechnology
- Cryobiology
Background:
- Apples (Malus spp.) are vital temperate fruit crops.
- Wild and cultivated apples offer valuable genes for breeding disease-resistant and climate-resilient cultivars.
- Apple cryobiology research has advanced significantly over 30 years.
Purpose of the Study:
- To review progress in apple cryopreservation and cryotherapy.
- To highlight the role of these techniques in preserving genetic resources and improving plant health.
- To discuss their contribution to the sustainable development of the apple industry.
Main Methods:
- Successful cryopreservation of various apple explants (pollen, seeds, buds, shoot tips).
- Establishment of large Malus germplasm cryobanks.
- Application of cryotherapy for virus eradication in apple plants.
Main Results:
- Cryopreservation has secured valuable genes for breeding elite apple cultivars and rootstocks.
- Cryotherapy has proven effective in producing virus-free apple plants.
- Significant progress in cryopreservation techniques has been achieved for Malus species.
Conclusions:
- Cryopreservation is crucial for conserving valuable apple genetic resources for future breeding.
- Cryotherapy significantly aids in producing healthy, virus-free apple plants.
- These biotechnological approaches are essential for the sustainable development of the global apple industry.
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