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A rhodamine-triazole fluorescent chemodosimeter for Cu2+ detection and its application in bioimaging
Kanokorn Wechakorn1,2, Samran Prabpai1,2, Kanoknetr Suksen3
1Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
Abstract:
A rhodamine-based fluorescent chemodosimeter rhodamine hydrazide-triazole (RHT) tethered with a triazole moiety was developed for Cu2+ detection. In aqueous medium, the RHT probe exhibited high selectivity and sensitivity toward Cu2+ among other metal ions. The addition of Cu2+ triggered a fluorescence emission of RHT by 384-fold (Φ = 0.33) based on a ring-opening process and a subsequent hydrolysis reaction. Moreover, RHT also showed a selective colorimetric response toward Cu2+ from colorless solution to pink, readily observed with the naked eye. The limit of detection of RHT for Cu2+ was calculated to be 1 nM (0.06 ppb). RHT was successfully demonstrated to detect Cu2+ in Chang liver cells by confocal fluorescence microscopy.
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