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Updated: Feb 15, 2026

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
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Developing a flexible, high-efficiency Agrobacterium-mediated sorghum transformation system with broad application.

Ping Che1, Ajith Anand1, Emily Wu1

  • 1DuPont Pioneer, Johnston, IA, USA.

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|January 13, 2018
PubMed
Summary

This study introduces an efficient Agrobacterium-mediated transformation system for sorghum, overcoming previous limitations in genetic improvement. The new method enhances sorghum transformation, enabling advancements in crop development and gene editing for this vital cereal crop.

Keywords:
AgrobacteriumAfrica sorghum varietiesCRISPR/Cas9genome modificationsorghum transformationternary vector

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Area of Science:

  • Plant Biotechnology
  • Agricultural Science
  • Molecular Biology

Background:

  • Sorghum is a crucial global cereal crop, vital for food security, especially in arid regions.
  • Limited sorghum genetic improvement is attributed to inefficient transformation systems.
  • Developing robust transformation methods is key to advancing sorghum breeding.

Purpose of the Study:

  • To develop an efficient Agrobacterium-mediated transformation system for sorghum.
  • To improve the genetic modification of elite and African sorghum varieties.
  • To enable CRISPR/Cas9 gene editing in sorghum.

Main Methods:

  • Utilized a ternary vector system with the pVIR accessory plasmid for Agrobacterium-mediated sorghum transformation.
  • Tested various selectable markers and evaluated regeneration frequencies and quality events in Tx430.
  • Applied the developed system to recalcitrant African sorghum varieties (Macia, Malisor 84-7, Tegemeo).

Main Results:

  • Achieved regeneration frequencies of 6% to 29% in Tx430.
  • Obtained 45% to 66% single-copy, backbone-free 'quality events'.
  • Successfully established transformation protocols for Macia, Malisor 84-7, and Tegemeo, and developed the first stable CRISPR/Cas9 gene knockouts in Tx430.

Conclusions:

  • The reported ternary vector system significantly enhances sorghum transformation efficiency.
  • This breakthrough facilitates genetic improvement and gene editing in diverse sorghum varieties, including African landraces.
  • The developed system holds great promise for accelerating sorghum crop development and addressing food security challenges.