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Updated: Feb 15, 2026

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
Published on: October 28, 2021
miRNA-20a upregulates TAK1 and increases proliferation in osteosarcoma cells
Guangke Yuan1,2, Yanqing Zhao2, Dongjin Wu1
1Department of Orthopedics, The Second Hospital of Shandong University, Shandong University, NO 247, Beiyuan Street, Jinan 250000, China.
Aim:
The aim of this study is to explore the function of miR-20a in osteosarcoma.
Materials & Methods:
miR-20a expression was measured by real-time PCR. miR-20a mimics, inhibitor and scramble siRNA were transfected into osteosarcoma cells to observe effects on colony formation and tumor growth. Moreover, relationships of miR-20a with TAK1 were investigated by western blot and luciferase activity.
Results:
We found that miR-20a was downregulated in osteosarcoma, and overexpression of miR-20a reduced colony formation and tumor growth. Furthermore, the data revealed that the function of miR-20a was probably exerted via targeting the TAK1 expression. Overexpression of miR-20a sensitizes the osteosarcoma cells to chemotherapeutic drugs.
Conclusion:
Our data identify the role of miR-20a in osteosarcoma growth, indicating its potential application in chemotherapy.
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